PCR with the fluorogenic DNA stain SYBR® Green I
نویسندگان
چکیده
منابع مشابه
Complex DNA melting profiles of small PCR products revealed using SYBR Green I.
1.Fire, A., S. Xu, M. K. Montgomery, S.A. Kostas, S.E. Driver, and C.C. Mello. 1998. Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature 391:806-811. 2.Sharp, P.A. 2001. RNA interference—2001. Genes Dev. 15:485-490. 3.Elbashir, S.M., W. Lendeckel, and T. Tuschl. 2001. RNA interference is mediated by 21and 22-nucleotide RNAs. Genes Dev. 15: 188-200. ...
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SYBR Green I (SG) is widely used in real-time PCR applications as an intercalating dye and is included in many commercially available kits at undisclosed concentrations. Binding of SG to double-stranded DNA is non-specific and additional testing, such as DNA melting curve analysis, is required to confirm the generation of a specific amplicon. The use of melt curve analysis eliminates the necess...
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SYBR Green I (SG) is widely used in real-time PCR applications as an intercalating dye and is included in many commercially available kits at undisclosed concentrations. Binding of SG to double-stranded DNA is non-speci®c and additional testing, such as DNA melting curve analysis, is required to con®rm the generation of a speci®c amplicon. The use of melt curve analysis eliminates the necessity...
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ژورنال
عنوان ژورنال: Technical Tips Online
سال: 2001
ISSN: 1366-2120
DOI: 10.1016/s1366-2120(08)70158-2